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目的:研究Th22细胞在骨髓增生异常综合征(MDS)患者外周血中的表达水平,并探讨其意义。方法:①用流式细胞术分别检测MDS较高危组、较低危组及正常对照组外周血中Th22细胞所占比例。②用酶联免疫吸附实验(ELISA)分别检测各组外周血中IL-22、TGF-β、TNF-α、IL-6的表达水平。③用半定量RT-PCR分别检测各组外周血中Th22细胞的相关转录因子IL-22 mRNA的表达水平,并逐层分析比较。结果:MDS较高危组、较低危组Th22细胞比例、IL-22、TNF-α、IL-6、TGF-β、IL-22 mRNA的表达水平分别为(0.53±0.13)%、(13.51±3.87)ng/L、(146.74±29.74)ng/L、(77.58±16.23)ng/L、(5.21±1.65)ng/L、0.29±0.06和(0.85±0.21)%、(16.13±4.82)ng/L、(182.34±37.55)ng/L、(95.62±20.28)ng/L、(7.46±2.17)ng/L、0.38±0.07,均低于正常对照组的(1.24±0.31)%、(19.72±6.55)ng/L、(238.32±50.41)ng/L、(137.95±27.08)ng/L、(9.65±2.78)ng/L、0.49±0.09,差异有统计学意义(P<0.05),MDS较低危组Th22细胞比例、IL-22、TNF-α、IL-6、TGF-β、IL-22 mRNA的表达水平均高于较高危组,差异有统计学意义(P<0.05)。结论:MDS患者体内Th22细胞数量减少,可能与MDS的发生发展呈负相关,提示MDS患者体内TNF-α、IL-6的表达减低及负向免疫细胞增多可能抑制Th22细胞的分化发育。
Objective: To investigate the expression of Th22 cells in peripheral blood of patients with myelodysplastic syndrome (MDS) and its significance. Methods: ① Flow cytometry was used to detect the proportion of Th22 cells in peripheral blood of MDS patients with higher risk group, lower risk group and normal control group respectively. ② The levels of IL-22, TGF-β, TNF-α and IL-6 in peripheral blood of each group were detected by enzyme linked immunosorbent assay (ELISA). ③ Semi-quantitative RT-PCR were used to detect the level of IL-22 mRNA expression in peripheral blood of Th22 cells. Results: The levels of Th22 cells, IL-22, TNF-α, IL-6, TGF-β and IL-22 mRNA in high risk group and low risk group were (0.53 ± 0.13)% and 3.87, ng / L, (147.74 ± 29.74) ng / L, (77.58 ± 16.23) ng / L, 5.21 ± 1.65 ng / L, 0.29 ± 0.06 and 0.85 ± 0.21, /L, (182.34 ± 37.55) ng / L, (95.62 ± 20.28) ng / L, (7.46 ± 2.17) ng / L, 0.38 ± 0.07, which were all lower than those in the normal control group (1.24 ± 0.31)% (P <0.05), and the difference was statistically significant (P <0.05). There was no significant difference between the two groups The expression levels of Th22 cells, IL-22, TNF-α, IL-6, TGF-β and IL-22 mRNA in the lower risk group were higher than those in the higher risk group, the difference was statistically significant (P <0.05). Conclusion: The decrease of Th22 cells in MDS patients may be negatively correlated with the occurrence and development of MDS. It suggests that the decrease of TNF-α and IL-6 expression and the increase of negative immune cells in MDS patients may inhibit the differentiation and development of Th22 cells.