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目的建立骨筋丸胶囊中马钱子的含量检测方法。方法采用SinochromODS-BP(4.6mm×250mm,粒径5μm)色谱柱,甲醇∶水∶三乙胺(55∶45∶0.135)为流动相,流速1.0ml/min,检测波长254nm,柱温40℃。结果番木鳖碱(士的宁)在8.8￣88.0μg/ml范围内线性关系良好(r=0.9998),平均回收率为99.11%[相对标准偏差(RSD)=2.29%];马钱子碱在10.2￣102.0μg/ml范围内线性关系良好(r=0.9999),平均回收率为99.31%(RSD=2.72%)。结论该方法专属性强、准确、可靠,可方便地同时测定士的宁和马钱子碱的含量,有效地控制制剂中马钱子的量。
Objective To establish a method for determining the content of Strychnos nucifera in Gujin Pill Capsule. Methods The chromatographic column was a Sinochrom ODS-BP (4.6 mm×250 mm, particle size 5 μm). The mobile phase consisted of methanol:water:triethylamine (55:45:0.135). The flow rate was 1.0 ml/min. The detection wavelength was 254 nm. The column temperature was 40°C. . Results The calibration curve of strychnine (strathrine) was linear in the range of 8.8 to 88.0 μg/ml (r=0.9998). The average recovery was 99.11% [RSD=2.29%]; strychnine The linearity was good in the range of 10.2 to 102.0 μg/ml (r=0.9999). The average recovery was 99.31% (RSD=2.72%). Conclusion This method is highly specific, accurate and reliable, and can be used to determine the content of strychnine and strychnine at the same time, and effectively control the amount of strychnine in the preparation.