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对柯萨奇B4病毒(CVB4)衣壳蛋白VPl保守区的亲水性和二级结构进行分析和预测,选择可能代表优势抗原表位的肽段进行合成(VPl-l肽,RIYF KPKHVK AYV),并截取了该肽段的前12个残基(VPI-2肽)用同样方法进行合成.用VP1-1肽免疫家兔制备出高效价抗体,应用酶联免疫吸附法(ELISA)检测,这些抗体与CVBI-6病毒均有结合反应,VP1-1肽与型特异性CVB1-6抗体均有良好的结合反应,表明VP1-1肽是CVB的共同抗原表位.用VP1-1肽检测心肌炎患者血清CVBIgM抗体,阳性率在40%左右.
The hydrophilicity and secondary structure of VP1 conserved region of Coxsackievirus B4 virus (CVB4) capsid protein were analyzed and predicted. Peptides that could represent the dominant epitopes were selected for synthesis (VP1-1 peptide, RIYF KPKHVK AYV) , And the first 12 residues (VPI-2 peptide) of the peptide were intercepted and synthesized in the same way.High titer antibody was prepared by immunization of rabbit with VP1-1 peptide and detected by enzyme-linked immunosorbent assay (ELISA) Both of these antibodies had a binding reaction with CVBI-6 virus, and both VP1-1 peptide and type-specific CVB1-6 antibody showed a good binding reaction, indicating that VP1-1 peptide is a common antigen epitope of CVB. Myocarditis serum CVBIgM antibody, the positive rate of about 40%.