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目的:考察黄药子二萜内酯成分体外实验中对肝细胞的毒性。方法:MTT法检测了黄药子二萜内酯成分对人正常肝细胞株细胞活性的影响。利用酶活法检测了肝酶在黄药子二萜内酯成分作用下细胞内释放水平,从而进一步确证其毒性。分别利用荧光比色法和流式细胞仪荧光法检测黄药子二萜内酯成分作用下细胞内谷胱甘肽和活性氧水平。结果:黄药子二萜内酯对肝细胞具有强烈的毒性作用,在黄药子二萜内酯成分作用下细胞内测谷胱甘肽下降同活性氧的上升呈相关性,而NAC能明显抑制二萜内酯对肝细胞的毒性作用。结论:黄药子二萜内酯体外对肝细胞具有毒性作用,其机制可能与该成分引起肝细胞氧化应激有关。
Objective: To investigate the cytotoxicity of xanthan gum diterpenoid lactone to hepatocytes in vitro. Methods: MTT assay was used to determine the effect of xanthonic diterpene lactones on the cell viability of human normal hepatocytes. The enzyme activity assay was used to detect the intracellular release of liver enzyme under the action of xanthophyll diterpene lactone, which further confirmed its toxicity. Fluorescence colorimetry and flow cytometry were used to detect intracellular glutathione and reactive oxygen species under the action of xanthan gum diterpene lactones. Results: Xanthium diterpene lactone has a strong toxic effect on hepatocytes. Under the action of xanthogenic diterpene lactones, intracellular glutathione degradation was correlated with the rise of reactive oxygen species. NAC could significantly inhibit the intracellular Ester on the toxic effects of liver cells. CONCLUSION: Xanthium diterpenoid lactone has a toxic effect on hepatocytes in vitro, which may be related to the oxidative stress induced by this component.