论文部分内容阅读
以长白落叶松采穗圆和扦插床的稳条为材料．应用间接酶联免疫吸附（ELISA）测定法。研究采穗母树在不同发育时期、插条在生根过程中的内源激素变化。结果表明：长白落叶松采穗母树和插条在不同时期的内源激素含量均有显著差异．插穗自扦插后GA4的含量显著增加．IPA的含量也高于对照；ABA的含量没有增加，反而有所下降。GA4、IPA在插条的愈伤组织形成及不定根产生过程中起促进作用。采穗母树的内源激素变化与插条的内源激素变化相反。根据采稳母树各生长期的激素变化及其它条件．可以确定最佳采穗期为7月初。
Larch Changbai spruce and the stability of the cuttings for the material. Indirect Enzyme-linked Immunosorbent Assay (ELISA) assays were applied. To study the changes of endogenous hormones of cuttings during rooting at different developmental stages. The results showed that there were significant differences in endogenous hormones content between the planted trees and cuttings of Larix olgensis at different periods. Cuttings from cutting GA4 content significantly increased. IPA content is also higher than the control; ABA content did not increase, but declined. GA4 and IPA play an important role in callus formation and adventitious root formation of cuttings. The changes of endogenous hormones in spikelets were opposite to the changes of endogenous hormones in cuttings. According to stabilize the growth of the mother tree hormone changes and other conditions. Can determine the optimal heading date early July.