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目的:观察不同代数RAW264.7细胞形成破骨细胞样细胞的能力是否改变。方法:以细胞核因子κB受体激活物的配体(receptor activator of NF-κB ligand,RANKL)为诱导剂,选用第6、9、13、18、24代RAW264.7细胞在体外诱导6天,固定细胞行抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,TRAP)染色,计数阳性细胞数及总细胞数。结果:第6、9、13、18、24代细胞均有TRAP染色阳性细胞;第6、9、13、18代细胞的阳性率无显著差异(P>0.05),而第24代与第6、9、13、18代细胞的阳性率显著降低(P<0.01)。结论:RANKL可以诱导RAW264.7细胞形成破骨细胞样细胞;RAW264.7细胞经多次传代后分化为破骨细胞样细胞的能力明显减弱。
Objective: To observe whether the ability of different algebraic RAW264.7 cells to form osteoclast-like cells is changed. Methods: The RAW264.7 cells of 6th, 9th, 13th, 18th and 24th generations were induced in vitro by receptor activator of NF-κB ligand (RANKL) The fixed cells were stained with tartrate-resistant acid phosphatase (TRAP) for counting the number of positive cells and the total number of cells. Results: The positive cells of TRAP staining were detected on the 6th, 9th, 13th, 18th and 24th generation of cells. The positive expression of the 6,9,13,18th generation cells showed no significant difference (P> 0.05) , The positive rate of 9th, 13th and 18th generation cells was significantly decreased (P <0.01). CONCLUSION: RANKL can induce RAW264.7 cells to form osteoclast-like cells. The ability of RAW264.7 cells to differentiate into osteoclast-like cells after multiple passages is obviously weakened.