论文部分内容阅读
探讨特发性血小板减少性紫癜（ＩＴＰ）患儿免疫功能变化。方法采用双抗体夹心ＥＬＩＳＡ法对ＩＴＰ患儿进行了血清可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）测定和ＡＰＡＡＰ法检测Ｔ淋巴细胞亚群。结果ＩＴＰ患儿血清ｓＩＬ－２Ｒ、ＣＤ８显著升高，ＣＤ４／ＣＤ８显著下降（Ｐ＜０．０１），且ｓＩＬ－２Ｒ和ＣＤ４／ＣＤ８呈负相关（ｒ＝－０．５８１，Ｐ＜０．０１）；缓解期ｓＩＬ－２Ｒ明显下降，基本接近正常水平，而ＣＤ８、ＣＤ４／ＣＤ８比值较急性期变化不明显。结论提示ｓＩＬ－２Ｒ和Ｔ淋巴细胞亚群的检测对ＩＴＰ患儿病情的监测有重要意义
To investigate the changes of immune function in children with idiopathic thrombocytopenic purpura (ITP). Methods Serum soluble interleukin 2 receptor (sIL-2R) and APAAP were used to detect T lymphocyte subsets in children with ITP by double antibody sandwich ELISA. Results Serum levels of sIL-2R and CD8 in ITP children were significantly higher than those in children with ITP (P <0.01). There was a negative correlation between sIL-2R and CD4 / CD8 (r = -0.581, P <0. 01). The sIL-2R level in the remission period was significantly lower than the normal level, but the ratio of CD8 and CD4 / CD8 was not significantly changed in acute stage. The results suggest that the detection of sIL-2R and T lymphocyte subsets is of great importance for the monitoring of ITP children’s condition