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以金丝小枣当年生枣头为外植体、以改良ＭＳ培养基为基本培养基，附加不同浓度的２，４—Ｄ、 ＩＢＡ诱导愈伤组织产生。 ２，４—Ｄ易诱导出疏松的Ⅱ型愈伤组织，ＩＢＡ则有利于致密的Ⅰ型愈伤组织形成，黑暗条件下诱导愈伤组织比光照条件下更有效。愈伤组织继代繁殖时，２，４—Ｄ的浓度影响了愈伤组织类型和染色体的数目变异。 两种类型愈伤组织再分化能力不同，Ⅱ型几乎没有分化出芽的能力、而Ⅰ型在相对高浓度６ＢＡ存在下分化出不定芽，同时在ＩＢＡ０．２＋６ＢＡ１的ＭＳ上诱导出大量胚状体。 选择了３０个芽丛再生植株元性系进行继代繁殖，０．３ｐｐｍＩＢＡ最有利于生根。这３０个植株无性系中有１个四倍体，２９个为二倍体。
The jujube head was used as explants and the modified MS medium was taken as the basic medium. Different concentrations of 2,4-D and IBA were added to induce the callus formation. 2,4-D could induce loose type II callus, IBA was conducive to the formation of compact type I callus, callus induction under dark conditions more effective than light conditions. When callus subcultured, the concentration of 2,4-D affected the callus type and the number of chromosome variations. The two types of callus had different ability of redifferentiation. Type II had almost no ability to differentiate into buds. However, Type Ⅰ could differentiate into adventitious buds in the presence of relatively high concentration of 6BA. Meanwhile, a large number of embryoid bodies were induced on MS of IBA0.2 + 6BA1. Thirty germplasm regeneration systems were selected for reproductive succession, and 0.3 ppmIBA was the best for rooting. One of the 30 plant clones had one tetraploid and 29 diploid.