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目的:建立一种应用气相色谱仪-氢火焰离子化检测器定量测定保健食品中二十八烷醇含量的简便方法。方法:样品先经混合溶液提取,再利用Silyl-991衍生试剂对提取物进行衍生净化,然后用正己烷做为提取液,加水除杂,最后利用气相色谱仪的毛细管色谱柱和氢火焰离子化检测器对提取液进行分离和检测。结果:利用该方法,二十八烷醇与杂质可以得到良好分离.在0.32μg/ml～160.00μg/ml的线性范围内,相关系数R2=0.9989,最低检出量为0.57 ng,当样品添加浓度为3.20 mg/kg～32.00 mg/kg时,方法回收率范围在83.13%～100.63%之间。结论:该方法操作简便,灵敏度高,重现性和选择性好,分离效果良好,最低检出量和回收率均符合微量分析的要求,是检测食品中二十八烷醇的有效定性、定量方法。
Objective: To establish a simple and convenient method for the quantitative determination of octacosanol in health food by gas chromatography with hydrogen flame ionization detector. Methods: The samples were firstly extracted by mixed solution, and then the extract was purified by using Silyl-991 derivatization reagent. Then, the extract was extracted with n-hexane and water was added to remove impurities. Finally, capillary gas chromatography and flame ionization Detector to extract the separation and detection. Results: The elution fraction of octacosanol and impurities can be well separated.With the linear range of 0.32μg / ml ~ 160.00μg / ml, the correlation coefficient R2 = 0.9989 and the minimum detectable amount was 0.57ng. When the sample was added The recovery ranged from 83.13% to 100.63% at the concentration of 3.20 mg / kg to 32.00 mg / kg. Conclusion: The method is simple, sensitive, reproducible and selective, the separation effect is good, the minimum detection and recovery rates are in line with the requirements of trace analysis, is the detection of food qualitative analysis of octacosanol, quantitative method.