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为了进一步探讨粘附分子CD44在血细胞的分布特点及与免疫功能的关系 ,利用荧光双染色—流式细胞仪技术检测各种血细胞的CD44分子的表达 ,利用3H_TdR掺入法检测T细胞的DNA合成。结果显示 :除血小板CD44分子的表达为阴性、胸腺细胞为弱阳性外 ,其他所有血细胞CD44分子的表达(包括红细胞、中性粒细胞、单核细胞、干细胞、T淋巴细胞、B淋巴细胞和NK细胞)均为阳性。单独抗CD3抗体可明显增强T细胞的DNA合成 ;但协同抗CD44抗体后 ,T细胞的DNA合成被明显抑制。单独抗CD44抗体对IL -2激活的T细胞DNA合成无明显影响 ;但协同抗CD2抗体后 ,可明显增强抗CD2抗体对IL -2激活的T细胞DNA合成的抑制。
To further investigate the distribution of CD44 in blood cells and its relationship with immune function, the expression of CD44 on various blood cells was detected by fluorescence double staining and flow cytometry. The DNA synthesis of T cells was detected by 3H_TdR incorporation . The results showed that in addition to the expression of platelet CD44 molecule was negative, thymus cells were weakly positive, the expression of CD44 in all other blood cells (including red blood cells, neutrophils, monocytes, stem cells, T lymphocytes, B lymphocytes and NK Cells) are positive. Anti-CD3 antibody alone can significantly enhance T cell DNA synthesis; but synergistic with anti-CD44 antibody, T cell DNA synthesis was significantly inhibited. Anti-CD44 antibody alone had no significant effect on DNA synthesis of IL-2 activated T cells; however, synergistic anti-CD2 antibody significantly enhanced anti-CD2 antibody inhibition of IL-2-activated T cell DNA synthesis.