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由于受培养条件的限制,受精卵在体外发育欠佳,特别是在2细咆期出现发育阻滞。联合培养是指将单层细胞作为营养细胞与人类胚胎一起培养,模拟体内发育。用于联合培养的细胞有多种,方法亦不同,如将人输卵管壶腹部细胞Vero细胞、胎牛子宫纤维细胞等制成单层细胞用来培养人胚胎能改善胚胎发育,提高妊娠率等。
Due to the limitations of culture conditions, fertilized eggs in poorly developed in vitro, especially in the 2 roar growth retardation. Co-culture means that monolayer cells are cultured together with human embryos as vegetative cells to simulate in vivo development. For the co-culture of a variety of cells, methods are also different, such as human fallopian tube ampulla cells Vero cells, fetal bovine uterine fibroblasts and other monolayer cells used to raise human embryos can improve embryonic development and improve pregnancy rates.