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为明确广西蓝莓产区叶斑病的致病菌种类,通过组织分离和纯化、致病性测定,并结合其产孢细胞,分生孢子的颜色、大小、附属丝等形态学特征和分子生物学特性对该病原菌进行鉴定。结果表明,该病原菌可致蓝莓叶片产生褐色病斑,边缘有深褐色晕圈,后期产生黑色分生孢子盘。该菌菌落呈白色,背面乳白色至淡黄色,产孢细胞无色透明,圆棒状。分生孢子(21.5~25.8)μm×(5.5~7.5)μm,中间3个色胞同为橄榄色,具2~3根顶端附属丝,长11.4~21.6μm。结合系统发育分析确定该病原菌为Pestalotiopsis trachicarpicola。是Pestalotiopsis trachicarpicola引起蓝莓叶斑病在国内的首次报道。
In order to clarify the pathogens of leaf spot in blueberry producing areas of Guangxi, through the tissue separation, purification and pathogenicity determination, the morphological characteristics and molecular biology of spore cells, conidia, conidia, Learn characteristics of the pathogen identification. The results showed that the pathogen could cause brown spot on blueberry leaves, dark brown halo on the edge and black conidial disk at the later stage. The colonies were white colonies, the back of milky white to light, spore cells colorless, transparent, round rod. Conidia (21.5 ~ 25.8) μm × (5.5 ~ 7.5) μm, the middle of the three color cells with olive, with 2 to 3 apical appendages, length 11.4 ~ 21.6μm. Combined with phylogenetic analysis, the pathogen was identified as Pestalotiopsis trachicarpicola. Is the first report of Pestalotiopsis trachicarpicola causing blueberry leaf spot in China.