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以尖山和弥渡种子为主区,IAA、GA3和IBA浸种采用L16(45)、GA3和IBA采用L9(34)的正交设计的水平组合为副区开展裂区下的正交实验,了解以上因素和水平及其组合对云南松幼苗胚轴和根生长的影响。结果表明种子产地和外源激素均影响幼苗的胚轴和根生长,激素处理的根都较对照的长,胚轴则比对照的短。种子产地和实验因素的组合不同,影响胚轴和根长的主导因子也不同,实验因子的两两交互作用对胚轴和根长具有极显著或显著的差异影响(P<0.01或P<0.05)。L16(45)实验中尖山种子培育幼苗实际胚轴和根长最长的分别是0.10与0.10 g·L-1的GA3及IBA浸种和对照的(3.9 cm和3.2 cm);弥渡的则为0.15、0.15、0.05 g·L-1的IAA、GA3、IBA和0.10、0.10 g·L-1的GA3、IBA浸种的(3.3 cm和2.6 cm)。L9(34)中,尖山种子由GA3和IBA浓度为0.05、0.05和0.15、0.05 g·L-1浸种的幼苗胚轴和根长最长,达3.8 cm和3.3 cm;弥渡的则来源于2种激素浓度为0.15、0.10 g·L-1和0.05、0.15 g·L-1的(3.3 cm和2.8 cm)。
Taking Jianshan and Midu seeds as the main area, the IAA, GA3 and IBA soaking seeds were used for the orthogonal experiment under the split zone using L16 (45), GA3 and IBA orthogonal design of L9 (34) Effect of Factors and Levels and Their Combination on Hypocotyl and Root Growth of Pinus yunnanensis Seedlings. The results showed that seed production and exogenous hormones all affected the hypocotyl and root growth of seedlings. The roots of hormone treatments were longer than the control, but the hypocotyls were shorter than the control. The dominant factors affecting the hypocotyl and the root length were also different with the combination of the origin of the seeds and the experimental factors. Any two-way interaction of the experimental factors had a significant or significant difference in the hypocotyl and the root length (P <0.01 or P <0.05 ). In L16 (45) experiment, the actual hypocotyl and root length of Jianshan seed culture seedlings were 0.10 and 0.10 g · L-1 GA3 and IBA soaking and control (3.9 cm and 3.2 cm respectively) 0.15, 0.15 and 0.05 g · L -1 IAA, GA 3, IBA and 0.10, 0.10 g · L -1 GA 3, soaked IBA (3.3 cm and 2.6 cm). In L9 (34), the seedlings of Jianshan seed soaked by GA3 and IBA at the concentrations of 0.05, 0.05 and 0.15, 0.05 and 0.05 g · L-1 had the longest hypocotyl and root length of 3.8 cm and 3.3 cm, respectively. The concentrations of two hormones were 0.15,0.10 g · L-1 and 0.05,0.15 g · L-1 (3.3 cm and 2.8 cm) respectively.