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目的 了解预变性神经移植修复神经缺损较新鲜神经移植修复效果良好的机制 ,并研究预变性神经段分子生物学变化。方法 选用 3 6只SD大鼠 ,将大鼠双侧上肢尺神经在腋部切断 ,远断端埋入肱二头肌中。术后当天、3d、1周、2周、3周、4周 ( 1～ 6组 )取材 ,应用rt PCR ,SouthernBlot的方法检测不同时间段的预变性神经中BDNF和CNTF的变化。结果 预变性神经中BDNF和CNTF的变化有明确的特点。预变 1周 (第 3组 )内BDNF的含量最高 ,以后 ,在较长的时间内 ( 14～ 2 8d ,即第 4,5 ,6组 )维持在较高水平。CNTF的含量有两个高峰 ,一个高峰是在预变后第 3天 (第 2组〕 ,第二个高峰是在预变后 3周 (第 5组 ) ,此后CNTF又下降。结论 Schwanncells在不同时间段的预变性神经段分泌不等量BDNF和CNTF ,两者的协同作用可促进神经再生。
OBJECTIVE: To investigate the mechanism of repairing nerve defects by repairing nerve defects and comparing them with fresh nerve grafts and to study the molecular biology changes of pre-degeneration nerve segments. Methods Thirty-six SD rats were selected, and the bilateral upper limb ulnar nerves of rats were cut off in the armpit and buried in the biceps femoris. The changes of BDNF and CNTF in pre-denervated nerve at different time points were detected by rt PCR and Southern blotting on the day after surgery, 3d, 1 week, 2 weeks, 3 weeks and 4 weeks (groups 1 to 6). Results The changes of BDNF and CNTF in the pre-denervated nerve have definite characteristics. The content of BDNF was the highest in the first week (group 3), and remained high after a long period of time (14 ~ 28 days, ie, groups 4, 5 and 6). CNTF content had two peaks, one peak at 3 days after pre-change (group 2), the second peak at 3 weeks after pre-change (group 5), then CNTF decreased again.Conclusion Schwanncells in different Predegenerative nerve segments of the period of secrete an unequal amount of BDNF and CNTF, the synergistic effect of both can promote nerve regeneration.