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A novel surface treatment method using 3-aminopropyltriethoxysilane (AM PTES),was developed to immobilize the fluorescein molecule on nano-HAP (nanometer hydroxyapatite) powders.By pretreating the nano-HAP powders surface with AMPTES,fluorescein,chosen on the basis of the chemical structure of the nano- HAP powders,could be bound to the nano-HAP powders surface.The chemical compositions of nano-HAP before and after being labeled were characterized by Fourier transform infrared spectroscopy(FTIR) and X-ray photoelectron spectroscopy (XPS).The morphology,phase composition,and the fluorescence characteristics of the nano-HAP powders with and without staining were also investigated.The FTIR and XPS results revealed that fluorescein had been successfully immobilized on the surface of AMPTES-bound nano-HAP powders via the acylamide bond formation between the -COOH of fluorescein and the -NH_2 of AMPTES.The labeled nano-HAP powders possessed strong fluorescent intensity with a little deviation from the maximum emission wavelength of fluorescein.But the morphology and phase composition had no obvious alteration.Under fluorescence microscopy,the labeled nano-HAP powders,even after 24 h cell incubation,exhibited strong fluorescence.
A novel surface treatment method using 3-aminopropyltriethoxysilane (AM PTES), was developed to immobilize the fluorescein molecule on nano-HAP (nanometer hydroxyapatite) powders. By pretreating the nano-HAP powders surface with AMPTES, fluorescein, chosen on the basis of the chemical structure of the nano-HAP powders, could be bound to the nano-HAP powders surface. The chemical compositions of nano-HAP before and after being labeled were were by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy ) The morphology, phase composition, and the fluorescence characteristics of the nano-HAP powders with and without staining were also investigated. The FTIR and XPS results revealed that fluorescein had been successfully immobilized on the surface of AMPTES-bound nano-HAP powders via the acylamide bond formation between the -COOH of fluorescein and the -NH_2 of AMPTES. labeled nano-HAP powders possessed strong fluorescent intensity with a little deviatio n from the maximum emission wavelength of fluorescein.But the morphology and phase composition had no obvious alteration .Under fluorescence microscopy, the labeled nano-HAP powders, even after 24 h cell incubation, showed strong fluorescence.