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目的了解宁波市Rh阴性献血者MN、Rh、Kidd、Lewis血型基因频率遗传学多样性。方法对250名Rh阴性献血者MN、Rh、JK、Lea血型进行血清学及分子生物学检验,分析其基因频率。结果Rh血型分布为CCEe2例(0.8%),Ccee25例(10.0%),CcEE1例(0.4%),CcEe12例(4.8%),Ccee71例(28.4%),ccEE12例(4.8%),ccEe16例(6.4%),ccee111例(44.4%),未发现CCEE。MN血型分布为MN82例(32.8%),M102例(40.8%),N66例(26.4%),基因频率分别为53.20%和46.80%。Kidd血型分布为JK(a+b-)68例(27.2%),JK(a-b+)62例(24.8%),JK(a+b+)120例(48%),未发现JK(a-b-),基因频率分别JKa50.2%、JKb49.8%。Lewis血型分布为Le(a+b-)46例(18.4%),Le(a-b+)127例(50.8%),Le(a-b-)85例(34.0%),Le(a+b+)2例(0.8%),基因频率分别为Lea43.82%和Leb56.18%。结论宁波市Rh阴性献血者Rh、MN、Kidd、Lewis血型分布特征总体符合中国汉族人群的分布特征,有待进一步增加样本数量进行基因频率遗传学多样性研究。
Objective To understand the genetic diversity of MN, Rh, Kidd and Lewis genotypes in Rh-negative blood donors in Ningbo. Methods The genotypes of MN, Rh, JK and Lea in 250 Rh-negative blood donors were tested by serological and molecular biological methods and their gene frequencies were analyzed. Results Rh blood type distribution was CCEe2 (0.8%), Ccee25 (10.0%), CcEE1 (0.4%), CcEe12 (4.8%), Ccee71 (28.4%), ccEE12 (4.8%) and ccEe16 6.4%), ccee111 cases (44.4%), found no CCEE. The distribution of MN blood type was MN82 (32.8%), M102 (40.8%), N66 (26.4%), and the gene frequencies were 53.20% and 46.80% respectively. The distribution of Kidd blood type was JK (a + b-) 68 cases (27.2%), JK (a-b +) 62 cases (24.8%) and JK (a + b + ), Gene frequency were JKa50.2%, JKb49.8%. The distribution of Lewis blood type was 46 (18.4%) in Le (a + b-), 127 (50.8%) in Le (a-b), 85 (34.0%) in Le Cases (0.8%), gene frequency were Lea43.82% and Leb56.18%. Conclusion The distribution characteristics of Rh, MN, Kidd and Lewis in Rh-negative blood donors in Ningbo are generally in line with the distribution characteristics of Chinese Han population. The number of samples needs to be further increased to study the gene frequency and genetic diversity.