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目的采用手术移植法或逆流法加用多因素整合方法建立子宫内膜异位症气滞血瘀证大鼠模型,以期为研究子宫内膜异位症气滞血瘀证的病理机制和中西医结合治疗提供合适的动物模型。方法采用逆流法、腹腔种植和皮下种植3种方法建立大鼠子宫内膜异位症模型,采用药物加情志刺激等多因素干预方法造成气滞血瘀证候模型,观察模型大鼠的外部体征和行为学表现,在位和异位内膜的组织形态学表现,对子宫内膜异位症气滞血瘀证大鼠模型进行评价。结果移植物大体呈小囊状,色白透明或暗红,显微镜下小囊具有子宫内膜的基本组织结构,模型动物具有中医“气滞血瘀”的临床症状。各组之间异位内膜未发现有明显差异。结论气滞血瘀型子宫内膜异位症造模方法简便易行,周期短,成活率高,可应用于中医药研究。
Objective To establish a rat model of endometriosis qi stagnation and blood stasis by surgical transplantation or countercurrent plus multi-factor integration method in order to study the pathological mechanism of qi stagnation and blood stasis syndrome of endometriosis and Chinese and Western medicine Combine treatment to provide the appropriate animal model. Methods The rat endometriosis model was established by three methods: countercurrent, intraperitoneal implantation and subcutaneous implantation. The model of qi stagnation and blood stasis syndrome was induced by multi-factor intervention such as drug plus emotional stimulation. The external appearance of model rats Signs and behavioral findings, eutopic and ectopic endometrial histopathological manifestations, evaluation of endometriosis qi stagnation and blood stasis syndrome rat model. Results The implants were mostly cystic, transparent and dark red in color. The microscopic vesicles had the basic structure of the endometrium. The model animals had the clinical symptoms of TCM “qi stagnation and blood stasis”. Ectopic endometrium between the groups did not find significant differences. Conclusion Qi stagnation and blood stasis type of endometriosis modeling method is simple and easy, short cycle, high survival rate, can be applied to traditional Chinese medicine research.