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目的 :探讨丹肠内营养对家兔肠道的保护作用。方法 :4 4只 10周龄近交系纯种大耳白幼兔 ,随机分为正常对照组、内毒素攻击组、能全力组和丹参肠内营养组 ,实验期为 10 d。实验结束时观察动物的体质量、血清白蛋白、超氧化物歧化酶 (SOD)、丙二醛 (MDA )、内毒素、小肠黏膜可溶性 Ig A、外周血淋巴细胞 DNA损伤等指标的变化。结果 :丹参肠内营养组血清内毒素水平为 (0 .5 3± 0 .2 4 ) Ek U/ L,明显低于内毒素攻击组的(2 .33± 0 .4 1) Ek U/ L(P<0 .0 1) ;而小肠黏膜 s Ig A(1.6 4± 0 .6 5 )较内毒素攻击组 (1.2 5± 0 .2 9)有升高趋势 ;血清 MDA水平为 (0 .5 32± 0 .197) μm ol/ L,也显著低于内毒素攻击组 (0 .890± 0 .0 5 1) μm ol/ L(P<0 .0 1)。与对照组比较 ,丹参肠内营养组家兔外周血淋巴细胞 DNA移动距离显著减小 (P<0 .0 1)。结论 :丹参肠内营养可以通过降低机体氧化损伤 ,保护肠道黏膜细胞 ,维护肠屏障功能 ,减少内毒素的移位 ,从而阻止病程发展 ,减轻器官功能损害。
Objective : To investigate the protective effect of enteral nutrition on rabbit intestine. METHODS: Fourty-four inbred blue-bred rabbits of 10 weeks old were randomly divided into normal control group, endotoxin challenge group, full-strength group and Salvia miltiorrhiza enteral nutrition group. The experimental period was 10 days. At the end of the experiment, animals’ body weight, serum albumin, superoxide dismutase (SOD), malondialdehyde (MDA), endotoxin, soluble IgA of small intestinal mucosa, DNA damage of peripheral blood lymphocytes and other indicators were observed. Results: The serum endotoxin level in the enteral nutrition group was (0.53 ± 0.24) Ek U/L, which was significantly lower than that in the endotoxin challenge group (2.33 ± 0.41) Ek U/L. (P <0.01), while s Ig A (1.64 ± 0.65) in the intestinal mucosa was higher than that in the endotoxin challenge group (1.2 5 ± 0.29); serum MDA levels were (0. 5 32± 0 .197) μm ol/L, also significantly lower than the endotoxin challenge group (0.890±0.05) μmol/L (P<0.01). Compared with the control group, the distance of peripheral blood lymphocyte DNA migration was significantly reduced in the enteral nutrition group (P<0.01). Conclusion : Enteral nutrition of Salvia miltiorrhiza can reduce the oxidative damage of the body, protect the intestinal mucosal cells, maintain the function of the intestinal barrier, reduce the translocation of endotoxin, thus preventing the development of the disease and reducing the damage of the organ.