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[目的]筛选能高效降解三唑磷的菌株,并研究其降解特性。[方法]从三唑磷生产厂的曝气池活性污泥中分离到1株三唑磷降解菌C-Y106,并根据C-Y106的形态、生理生化特征和16SrRNA同源性序列分析进行了菌株鉴定,同时研究了不同营养的培养基对C-Y106降解三唑磷速率的影响。[结果]经鉴定,C-Y106为枯草芽孢杆菌。C-Y106能以三唑磷为唯一碳源、唯一氮源、唯一磷源生长,其中以三唑磷为唯一磷源时其降解速率最大,且在31℃、初始pH值为8.0、150r/min条件下,培养60h后,三唑磷降解率为76.8%。[结论]为三唑磷降解酶的分离纯化提供了理论依据。
[Objective] The research aimed to screen the strains that can degrade triazophos effectively and study its degradation characteristics. [Method] A strain of triazophos-degrading bacterium C-Y106 was isolated from aeration tank activated sludge of triazophos production plant. According to the morphological, physiological and biochemical characteristics and 16S rRNA homology sequence analysis of C-Y106, Strain identification, at the same time studied the different nutrients of C-Y106 degradation of triazophos rate. [Result] It was identified that C-Y106 was Bacillus subtilis. C-Y106 could grow with triazophos as sole carbon source, only nitrogen source and only phosphorus source, and its degradation rate was the highest when triazophos was the only phosphorus source. At 31 ℃, the initial pH was 8.0,150 r / min, the degradation rate of triazophos was 76.8% after 60h culture. [Conclusion] This provided the theoretical basis for the isolation and purification of triazophos-degrading enzyme.