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目的鉴别四川产亚大黄,并测定其大黄素、大黄酚的含量。方法采用显微鉴别、TLC法对药材进行鉴别,用HPLC法测定大黄素、大黄酚的含量。结果 TLC法鉴别亚大黄的专属性强;建立了HPLC测定疏枝大黄中大黄素、大黄酚含量的方法。结论所用方法操作简便、结果准确、重复性好,可用于四川产亚大黄的质量控制。
Objective To identify Sichuan production of rhubarb, and determine the emodin, chrysophanol content. Methods Microscopic identification and TLC method were used to identify the medicinal materials. The contents of emodin and chrysophanol were determined by HPLC. Results The specificity of TLC was identified by TLC. A method for the determination of emodin and chrysophanol in Radix Scutellariae by HPLC was established. Conclusion The method is simple, accurate, reproducible and can be used for quality control of rhubarb in Sichuan.