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目的:为初步掌握黑龙江省小兴安岭林区动物莱姆病分布情况。方法:于1995年7月用鼠笼捕获野鼠30只,采用间接免疫荧光抗体法调查鼠血清抗莱姆病螺旋体抗体。用BSK培养基从鼠脏器分离病原。结果:阳性共8只,阳性率26.67%;其中优势种属野鼠阳性率达31.36%;从棕背和花鼠各分离出1株莱姆病螺旋体。新分离株的单克隆抗体反应与国内分离株SR1相同,与美国B(31)有区别,对H(6831)呈阴性反应。结论:鼠型啮齿动物可能是莱姆病螺旋体的动物宿主,属野鼠则是重要动物宿主。
Objective: To initially grasp the distribution of animal Lyme disease in the Xiao-xing’anling forest region of Heilongjiang province. Methods: Thirty cats were captured by squirrel cage in July 1995, and anti-Lyme disease spirochetes were detected by indirect immunofluorescence antibody. Pathogens were isolated from murine organs using BSK medium. Results: A total of 8 were positive, the positive rate was 26.67%. The positive rate of the dominant species was 31.36%. A strain of Lyme disease was isolated from the brown back and the rat. The monoclonal antibody response of the new isolate was the same as that of the domestic isolate SR1, which was different from the American B (31) and negative to H (6831). CONCLUSION: Murine rodents may be the animal hosts for Borrelia burgdorferi, while the wild animals are important animal hosts.