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目的：探讨子宫颈鳞癌中ｐ２１、ｐ５３的表达。方法：应用免疫组织化学ＳＰ方法观察３例正常子宫颈组织、２８例子宫颈慢性炎组织和４５例子宫颈鳞癌组织中的增殖细胞核抗原（ＰＣＮＡ）、ｐ２１和ｐ５３的标记结果。结果：３例正常子宫颈组织中３种均阴性；２８例子宫颈炎ｐ２１、ｐ５３也均阴性，ＰＣＮＡ阳性率为２％。４５例子宫颈鳞癌组织中，ｐ２１、ｐ５３和ＰＣＮＡ阳性率分别为１６％、７％、９８％。ＰＣＮＡ、ｐ５３阳性均定位于瘤细胞核，ｐ２１定位于细胞膜。ｐ２１、ｐ５３和ＰＣＮＡ阳性率与子宫颈鳞癌的病理分级无关（Ｐ＞０．０５）；除ＰＣＮＡ（Ｐ＜０．０１）外，ｐ２１和ｐ５３与临床分期无明显关系（Ｐ＞０．０５）。结论：检测ｐ２１、ｐ５３和ＰＣＮＡ有助于评估子宫颈癌的预后。
Objective: To investigate the expression of p21 and p53 in cervical squamous cell carcinoma. Methods: Immunohistochemical SP method was used to detect the expression of PCNA, p21 and p53 in 3 cases of normal cervical tissue, 28 cases of chronic cervicitis and 45 cases of cervical squamous cell carcinoma. Results: Three of the three cases of normal cervical tissue were negative. 28 cases of cervicitis had negative p21 and p53, and the positive rate of PCNA was 2%. The positive rates of p21, p53 and PCNA in 45 cases of cervical squamous cell carcinoma were 16%, 7% and 98% respectively. PCNA, p53 positive are located in the tumor nucleus, p21 located in the cell membrane. The positive rates of p21, p53 and PCNA were not correlated with the pathological grade of cervical squamous cell carcinoma (P> 0.05). There was no significant correlation between the expression of p21, p53 and clinical stage except PCNA (P <0.05) ). CONCLUSIONS: The detection of p21, p53 and PCNA contributes to assess the prognosis of cervical cancer.