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应用分子系统发育学的方法,以浙江沿海一海地瓜的18S rDNA和COI片段序列为分子标记,自行设计引物,进行了克隆,结合来自GenBank中10种海参的18S rDNA和COI同源序列,用邻接法和最大简约法构建系统进化树,并结合形态学特征对它们的分类地位进行了探讨。初步分析结果,基于此海地瓜的各形态学的研究,可以认为它属于海参纲芋参目;鉴于18S rDNA基因的分析,此海地瓜与拟刺参遗传距离为0.017亲缘关系非常近,可以认为它属于海参纲;从COI基因的角度分析,它虽然与怀玉参在系统树上聚成一枝,但支持率很低;仅为49%,遗传距离比较大,为0.223,不足以作为分析的依据。
Using molecular phylogenetics, 18S rDNA and COI fragment sequences of a sea cucumber from Zhejiang Province were used as molecular markers. Primers were designed and cloned. 18S rDNA and COI homologous sequences from 10 sea cucumbers in GenBank were used to amplify Adjacency method and the largest parsimony method were used to construct the phylogenetic tree, and their morphological characteristics were discussed. Preliminary analysis of the results, based on this study of the morphological Haitian melon can be considered as belonging to the cucumber line taro; 18S rDNA gene analysis, this Haitian melon and cucumber sea cucumber genetic distance 0.017 is very close, we can think It belongs to the sea cucumber class. From the perspective of the COI gene, although it is clustered with Huayu ginseng in a systematic tree, the support rate is very low. Only 49% of the population has a large genetic distance of 0.223, which is not enough for analysis in accordance with.