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AIM:To investigate gene expression profiles of intergringenes in hepatocellular carcinoma(HCC)through theusage of Atlas Human Cancer Array membranes,semi-quantitative reverse transcription polymerase chainreaction(RT-PCR)and Northern blot,METHODS:Hybridization of cDNA array membrane wasperformed with α 32P-labeled cDNA probes synthesizedfrom RNA isolated from hepatocellular carcinoma andadjacent non-cirrhotic liver.AtlasImage,which is asoftware specific to array,was used to analyze theresult.RT-PCR of 24 pairs specimen and Northern blotof 4 pairs specimen were used to confirm the expressionpattern of some intergrin genes identified by Atlasarrays hybridization.RESULTS:Among 588 genes spotted in membrane,17genes were related to intergrin.Four genes were up-regulated,such as intergrin alpha8,betel,bete7 andbeta8 in HCC.Whereas there were no genes down-regulated in HCC.RT-PCR and Northern blot analysisof intergrin beta1 gene gave results consistent withcDNA array findings.CONCLUSION:Investigation of these intergrin genesshould help to disclose the molecular mechanism of thecell adhesion,invasive and metastasis of HCC.A fewgenes are reported to have changed in HCC for the firsttime.The quick and high-throughout method of profilinggene expression by cDNA array provides us overviewof key factors that may involved in HCC,and may findthe due of the study of HCC metastasis and moleculartargets of anti-metastasis therapy.The preciserelationship between the altered genes and HCC is amatter of further investigation.
AIM: To investigate gene expression profiles of intergrinses in hepatocellular carcinoma (HCC) through theusage of Atlas Human Cancer Array membranes, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Northern blot, METHODS: Hybridization of cDNA array membrane wasperformed with alpha 32P-labeled cDNA probe synthesized from RNA isolated from hepatocellular carcinoma and adjjacent non-cirrhotic liver. AtlasImage, which is as specific software to array, was used to analyze the results. RT-PCR of 24 pairs of specimens and Northern blot of 4 pairs of specimens were used to confirm the expressionpattern of some intergrin genes identified by Atlasarrays hybridization .RESULTS: Among 588 genes spotted in membrane, 17genes were related to intergrin. Four genes were up-regulated, such as intergrin alpha8, betel, bete7 andbeta8 in HCC. There are no genes down -regulated in HCC.RT-PCR and Northern blot analysis of intergrin beta1 gene gave results consistent with cDNA array findings. CONCLUSION: Inve stigation of these intergrin genesshould help to disclose the molecular mechanism of the cell adhesion, invasive and metastasis of HCC. A few genes were reported to have changed in HCC for the first time. The quick and high-throughout method of profiling gene expression by cDNA array provides us overview of key factors that may involve in HCC, and may find the due of the study of HCC metastasis and moleculartargets of anti-metastasis therapy. The precise relationship between the altered genes and HCC is amatter of further investigation.