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报道细胞分裂素和硝酸铵对欧洲云杉不定芽伸长过程的影响。采用5年生的欧洲云杉1个无性系不定芽丛来研究玉米素和硝酸铵对不定芽生长的影响。试验3种诱导类型(玉米素浓度分别为6·84、13·68、20·52μmol·L-1,激动素浓度0·69μmol·L-1,培养4周),玉米素最高浓度处理诱导的伸长芽数量最多,在4个无生长调节剂继代培养过程中,加入了1个硝酸铵浓度逐渐增加的继代培养过程。经不含生长调节剂的低浓度的硝酸铵(2·5mmol·L-1)培养基的继代培养后转入含有细胞分裂素的诱导培养基,下一个继代培养基补充的硝酸铵浓度分别为2·5、7·5、12·5、17·5mmol·L-1,8周(2个继代培养)后转入含有硝酸铵浓度为2·5mmol·L-1的培养基培养。硝酸铵浓度为7·5mmol·L-1的处理可形成数量最多的不定芽,这对于采用不同浓度的玉米素进行诱导的处理结果是一致的,但随着硝酸铵的浓度增至12·5mmol·L-1和17·5mmol·L-1时则不定芽的数量减少。欧洲云杉的胚轴外植体经1次或多次诱导后,细胞分裂素(BA、激动素、玉米素、2iP)促进了不定芽的分化和发育,其中BA由于可诱导产生大量的不定芽而成为最有效的细胞分裂素。玉米素虽然诱导不定芽相对弱一些,却在BA多次诱导后可促进芽的伸长。这对获得具有形成不定芽能力并可扩繁的芽丛非常重要。
The effects of cytokinin and ammonium nitrate on the adventitious bud elongation of spruce A 5-year-old European spruce adventitious shoots were used to study the effects of zeatin and ammonium nitrate on the growth of adventitious buds. Three types of induction (zeatin concentration of 6,84,13.68,22.2μmol·L-1, respectively, kinetin concentration of 0.69μmol·L-1, cultured for 4 weeks), induced by the highest concentration of zeatin The number of elongation buds was the largest. During the subculture of four non-growth regulators, one subculture was added with a gradually increasing ammonium nitrate concentration. Subculture with low concentrations of ammonium nitrate (2.5 mmol·L-1) without growth regulators, followed by induction with cytokinin-containing medium, with the next subculture medium supplemented with ammonium nitrate Respectively, after 2.5, 7.5, 12.5, 17.5 mmol·L-1, and 8 weeks (two subcultures), were cultured in a medium containing ammonium nitrate at a concentration of 2.5 mmol·L -1 . Treatment with ammonium nitrate at 7.5 mmol·L-1 resulted in the largest number of adventitious buds, which was consistent with induction with different concentrations of zeatin, but with increasing ammonium nitrate concentration to 12.5 mmol · L-1 and 17 · 5mmol · L-1 when the number of adventitious buds decreased. Adventitious bud differentiation and development are promoted by cytokinin (BA, kinetin, zeatin, 2iP) induced by one or more explants of European spruce (Hypocotyls), of which BA can induce a large amount of indefinite Bud and become the most effective cytokinin. Although zeatin induced adventitious buds relatively weak, but after many induction BA can promote bud elongation. This is important for obtaining bud clusters with the ability to form adventitious buds and to multiply.