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探讨过表达糖皮质激素诱导的亮氨酸拉链蛋白(glucocorticoid-induced leucine zipper,GILZ]对小鼠骨髓间充质干细胞(bone marrow-derived mesenchymal stem cells,BM-MSCs)成骨分化的影响。以逆转录病毒为介导,将绿色荧光蛋白(green fluorescent protein,GFP)基因和GILZ基因分别导入小鼠BM-MSCs中,得到MSC-GFP和MSC-GILZ细胞。以MSC-GFP细胞为对照,对MSC-GILZ细胞进行成骨分化诱导,结果显示GILZ过表达显著增加了碱性磷酸酶的表达和矿化骨节的形成。RT-PCR和Western blot结果显示GILZ过表达显著增加了Runx2和Osterix的mRNA和蛋白质表达。结果表明GILZ过表达对小鼠BM-MSCs成骨分化的增强,可能部分是通过促进成骨分化中两个关键的转录因子Runx2和Osterix的表达来实现的。
To investigate the effect of glucocorticoid-induced leucine zipper (GILZ) overexpression on osteogenic differentiation of mouse bone marrow-derived mesenchymal stem cells (BM-MSCs) Retrovirus-mediated transfection of green fluorescent protein (GFP) gene and GILZ gene into mouse BM-MSCs respectively to obtain MSC-GFP and MSC-GILZ cells.With MSC-GFP cells as control, MSC-GILZ cells induced osteogenic differentiation, the results showed that overexpression of GILZ significantly increased alkaline phosphatase expression and mineralization of joints formation.RT-PCR and Western blot results showed that GILZ overexpression significantly increased Runx2 and Osterix mRNA And protein expression.The results show that the overexpression of GILZ can enhance the osteogenic differentiation of mouse BM-MSCs in part through the promotion of the expression of two key transcription factors, Runx2 and Osterix, in osteogenic differentiation.