论文部分内容阅读
对江西省猕猴桃种质资源进行扩增片段长度多态性(AFLP)标记来鉴定分析.首先从64对引物筛选出4对引物,对31份种质材料的DNA进行检测,得到190个扩增基因位点,其中多态性位点179个,多态性比例为94.2 %,对31份种质材料的区分率达到100 %.然后,对扩增结果进行UPGMA聚类分析,谱系图显示,31份种质材料之间的相似系数在0.50 ~0.85之间,表明猕猴桃种质之间遗传关系相对来说不是很近.在相似系数0.56的水平上,可以将31份种质大致分为4个类群:净果组和斑果组为一类群;糙毛组为一类群;星毛组为一类群;中华猕猴桃和美味猕猴桃为一类群.从树状图中看,原为中华猕猴桃的一个变种的“赣猕5号”,现与中华猕猴桃并列,有对其作进一步分类方面深入研究的必要.本研究从分子角度鉴定分析了江西省猕猴桃种质资源及其遗传关系,其结果在很大程度上与传统分类是一致的,同时也为江西省猕猴桃种质资源分类学研究提供了新的证据.
Amplified fragment length polymorphism (AFLP) markers were used to identify and analyze the kiwifruit germplasm resources in Jiangxi Province.Firstly, four pairs of primers were screened from 64 pairs of primers to detect the DNA of 31 germplasm materials, and 190 amplified The number of polymorphic loci was 179, the percentage of polymorphism was 94.2%, and the discrimination rate was 100% for 31 germplasm materials.Then, the results of UPGMA clustering analysis showed that, The similarity coefficient between 31 germplasm ranged from 0.50 to 0.85, indicating that the genetic relationship between kiwifruit germplasm is not very close.When the similarity coefficient is 0.56, 31 germplasm can be divided into 4 A group of groups: the net fruit group and the group of spot fruit group as a group; the group of coarse hair as a group; the group of star hair group as a group; the Chinese kiwi fruit and delicious kiwi fruit as a group from the tree view, Variety “Gan kang 5” is now tied with Chinese kiwifruit and it is necessary to make further research on its further classification.In this study, the kiwifruit germplasm resources and their genetic relationship in Jiangxi Province were identified and analyzed from the molecular point of view, To a large extent, the traditional classification is the same, but also for the river Western kiwifruit germplasm taxonomic studies provide new evidence.