腐植酸与Fe(Ⅱ)-EDTA对体外培养的鸡胚软骨细胞合成分泌胶原蛋白类型及细胞骨架的影响

• 作 者: 陈瑞新 许善锦 王夔 赵翀
• 机 构: 北京医科大学药学院无机教研室,北京医科大学药学院无机教研室,北京医科大学药学院无机教研室,北京医科大学基础医学院免疫教研室 北京100083,北京100083,北京100083,北京100083
• 出 处: 科学通报
• 发表日期: 1995年19期
• 关键词: 软骨细胞 体外培养 Fe 细胞骨架 肌动蛋白骨架 形态发生 基因表达 胶原酶消化 胰蛋白酶消化 腐植酸

软骨细胞与其周围基质的钙化是长骨、椎骨等正常发育的关键.软骨的细胞正常分化表现为合成并分泌其周围基质的主要成分Ⅱ型胶原蛋白,软骨细胞分化异常,必然引起基质钙化异常,引发某类软骨性疾病.已有研究表明,细胞形态与细胞分化有着密切的关系,肌动蛋白骨架的变化可改变某种基因表达,我们在大骨节病病因的研究中发现,自由基能使软骨细胞形态发生改变.本文在离体条件下,在这方面进行了初步探讨.

Chondrocytes and their surrounding matrix calcification is the key to the normal development of long bones and vertebrae, etc. The normal differentiation of cartilage cells is characterized by the synthesis and secretion of the main components of its surrounding matrix type Ⅱ collagen, chondrocyte abnormalities, inevitably lead to abnormal matrix calcification, triggering Some types of cartilage disease.Researches have shown that cell morphology and cell differentiation are closely related to changes in the actin skeleton can change a certain gene expression, we found in the study of the cause of Kashin-Beck disease, free radicals can make cartilage Cell morphology changes.This article in vitro conditions, in this regard, a preliminary study.