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目的:检测直肠癌中血管内皮生长因子(VEGF)及血管生成素-2(Ang-2)的表达,探讨两者与直肠癌临床病理因素和肿瘤新生血管形成之间的关系。方法:采用免疫组化SP法检测60例直肠癌、30例直肠腺瘤及10例直肠正常黏膜组织中VEGF、Ang-2及FⅧ标记的微血管密度(MVD)表达,分析不同VEGF和Ang-2表达状态下直肠癌组织MVD的变化。结果:在直肠癌、直肠腺瘤及正常组织中VEGF的阳性表达率分别为71.7%(43/60)、43.3%(13/30)和30.0%(3/10),Ang-2的阳性表达率分别为73.3%(44/60)、46.7%(14/30)和30.0%(3/10)。VEGF的表达与患者的性别和年龄、肿瘤的形态和肿瘤部位、术前CEA情况及肿瘤分化程度无关(P>0.05),与直肠癌临床分期、淋巴结转移及远处转移相关(P<0.05);Ang-2的表达与患者的性别和年龄、肿瘤的形态和肿瘤部位、术前CEA情况及淋巴结转移无关(P>0.05),与肿瘤分化程度、直肠癌临床分期及远处转移相关,P<0.05。直肠癌组织中VEGF和Ang-2的表达呈正相关,r=0.290,P<0.05。VEGF在直肠癌组中染色阳性者其MVD较染色阴性者高(11.04±1.68 vs 7.66±1.22,t=7.53,P<0.05);Ang-2染色阳性者其MVD较染色阴性者高(10.97±1.73 vs 7.66±1.31,t=6.95,P<0.05)。结论:VEGF和Ang-2高表达与直肠癌发生发展及侵袭转移密切相关,在调节直肠癌的血管生成中可能起协同作用。
OBJECTIVE: To detect the expression of vascular endothelial growth factor (VEGF) and angiopoietin-2 (Ang-2) in rectal cancer and to explore the relationship between them and the clinicopathological factors and neovascularization of rectal cancer. Methods: The expressions of VEGF, Ang-2 and FⅧ in 60 rectal cancers, 30 rectal adenomas and 10 rectal mucosa were detected by immunohistochemical SP method. The expressions of VEGF and Ang-2 Changes of MVD in Rectal Cancer Tissues in Expressions. Results: The positive rates of VEGF expression in rectal cancer, rectal adenoma and normal tissues were 71.7% (43/60), 43.3% (13/30) and 30.0% (3/10), respectively. The positive expression of Ang-2 Rates were 73.3% (44/60), 46.7% (14/30) and 30.0% (3/10), respectively. The expression of VEGF correlated with the clinical stage, lymph node metastasis and distant metastasis of rectal cancer (P <0.05), but not with the gender and age of patients, tumor morphology and tumor location, preoperative CEA status and tumor differentiation (P> 0.05) ; The expression of Ang-2 was not related to the patient’s gender and age, tumor morphology and tumor location, preoperative CEA status and lymph node metastasis (P> 0.05), but also correlated with tumor differentiation, clinical stage and distant metastasis of P <0.05. The expression of VEGF and Ang-2 in rectal cancer tissues was positively correlated, r = 0.290, P <0.05. MVD in colorectal cancer group was higher than that in negative group (11.04 ± 1.68 vs 7.66 ± 1.22, t = 7.53, P <0.05). The positive rate of VEGF in Ang-2 positive group was higher than negative staining group (10.97 ± 1.73 vs 7.66 ± 1.31, t = 6.95, P <0.05). Conclusion: The high expression of VEGF and Ang-2 is closely related to the development, invasion and metastasis of rectal cancer and may play a synergistic role in regulating the angiogenesis of rectal cancer.