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目的观察富氢林格液(富氢液)对延迟复苏严重烧伤大鼠肠组织的保护作用。方法制备富氢液,采用随机数字表法将36只SD大鼠随机分为3组(每组12只),即假烫组、烫伤+普通林格液组和烫伤+富氢液组,在后两组大鼠背部造成占体表面积(TBSA)30%的Ⅲ度烫伤,并于烫伤后7 h、9 h、17 h,分别经腹腔补给相当于总补液体积1/2、1/4、1/4的普通林格液或富氢液,假烫组不补液。24 h后取小肠组织做H-E染色,观察病理变化,并检测肠组织中丙二醛(MDA)含量和髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)活性,以及促炎细胞因子IL-1β和TNF-α的含量。结果相对于普通林格液组,富氢液组小肠黏膜损伤减轻,肠组织MDA含量(P<0.05)及MPO和SOD活性均降低(P<0.01);促炎细胞因子IL-1β和TNF-α含量在富氢液组均显著低于普通林格液组(P<0.01)。结论富氢液对烧伤延迟复苏后肠组织具有保护作用,可能主要从两方面体现:一是降低大面积烧伤及延迟复苏所带来的氧化损伤;二是抑制延迟复苏大鼠肠组织促炎因子IL-1β和TNF-α的生成。
Objective To observe the protective effect of hydrogen-rich Ringer’s solution (hydrogen-rich liquid) on the intestinal tissue of severely burned rats delayed by resuscitation. Methods Forty-six SD rats were randomly divided into three groups (12 rats in each group) by random number table method, namely, fake-hot group, scald + ordinary Ringer’s solution group and scald + hydrogen- In the latter two groups, the third degree scalds of 30% of the body surface area (TBSA) were caused on the back of the rats, and the total volume of rehydration was 1 / 2,1 / 4 at 7 h, 9 h and 17 h after scald respectively. 1/4 ordinary Ringer’s solution or hydrogen-rich liquid, fake hot group is not rehydration. Twenty-four hours later, the small intestine tissue was taken for HE staining to observe the pathological changes. The content of malondialdehyde (MDA) and the activities of myeloperoxidase (MPO) and superoxide dismutase (SOD), as well as proinflammatory cells Factors IL-1β and TNF-α content. Results Compared with normal Ringer’s solution group, the intestinal mucosal injury alleviated, the content of MDA in intestinal tissue (P <0.05) and the activity of MPO and SOD decreased (P <0.01), and the levels of proinflammatory cytokines IL-1β and TNF- α content in hydrogen-rich liquid group were significantly lower than normal Ringer’s liquid group (P <0.01). Conclusion Hydrogen-rich solution has a protective effect on intestinal tissue after delayed resuscitation, which may be mainly reflected in two aspects: one is to reduce oxidative damage caused by extensive burns and delayed resuscitation; the other is to inhibit the delayed recovery of proinflammatory cytokines IL-1β and TNF-α production.