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为寻找从鱼油中分离高纯度二十碳五烯酸 (EPA)和二十二碳六烯酸 (DHA )的更有效方法 ,分别以 3,5 -二硝基苯甲酰氯和 2 -氯乙醇修饰的聚甲基丙烯酸甲酯(PMDVB)树脂微球 ,用作高效液相色谱 (HPL C)的固定相。均比修饰前的 PMDVB树脂微球分离效果好 ,比 Waters反相 C1 8键合硅胶柱分离因子大 ,柱容量高。以 3,5 -二硝基苯甲酰氯修饰的 PMDVB树脂效果最理想 ,以 2 -氯乙醇修饰的PMDVB树脂微球 ,造价低 ,工业上具有推广价值。作者对分离机理的研究表明 :缺电子基团 3,5 -二硝基苯甲酰氯与EPA、DHA形成电子转移络合物 ,电子转移络合物作用是一种弱的分子间作用力。
In order to find out a more effective method for separating high purity eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from fish oil, respectively, 3,5 - dinitrobenzoyl chloride and 2 - chloroethanol Modified Polymethylmethacrylate (PMDVB) resin microspheres were used as the stationary phase for High Performance Liquid Chromatography (HPL C). Compared with pre-modified PMDVB resin microspheres, the separation efficiency was better than that of Waters reversed-phase C1 8-bonded silica gel column with high column capacity. The PMDVB resin modified with 3,5 - dinitrobenzoyl chloride has the best effect. The PMDVB resin microspheres modified with 2 - chloroethanol have low cost and have the promotion value in industry. The study on the mechanism of separation showed that the electron - deficient 3,5 - dinitrobenzoyl chloride formed an electron transfer complex with EPA and DHA, and the electron transfer complex was a weak intermolecular force.