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目的:在甲流疫情结束后,分析两年以来锦州市甲型流感病毒的流行趋势和特点,为制定防控措施提供依据。方法:由两家哨点医院采集流感样病人的鼻咽拭子标本,接种于MDCK细胞进行分离培养,采用血凝抑制试验进行病毒亚型鉴定。结果:2010年10月-2012年3月,对1317份标本进行病毒分离及鉴定,共分离甲型病毒27株,阳性率达2.1%,其中新型H1N110株,季节性H3N217株;病毒检出高峰出现在2010年12月、2011年1月和2012年3月,阳性率分别为:6.9%、6.0%和4.3%;新型H1N1与季节性H3N2在中老年中分布最多,并且新型H1N1在各年龄段中发病率略高于季节性H3N2。结论:2010年10月-2012年3月锦州市甲型流感流行优势株为新型H1N1、季节性H3N2;中老年人流感阳性率较高,应加强对中老年人的监测,及时发现病毒变异情况。
OBJECTIVE: To analyze the epidemic trend and characteristics of Influenza A virus in Jinzhou City after two years of outbreak of Influenza A, and to provide the basis for making prevention and control measures. Methods: Nasopharyngeal swab samples of influenza-like patients were collected from two sentinel hospitals and inoculated on MDCK cells for isolation and culture. The virus subtype was identified by hemagglutination inhibition test. Results: From October 2010 to March 2012, 1317 samples were isolated and identified. A total of 27 strains of A virus were isolated, the positive rate was 2.1%, including the new H1N110 and seasonal H3N217 strains. The virus peak was detected Appeared in December 2010, January 2011 and March 2012, the positive rates were: 6.9%, 6.0% and 4.3%; new H1N1 and seasonal H3N2 in the distribution of the most elderly, and the new H1N1 in all ages The incidence in the segment was slightly higher than that of seasonal H3N2. Conclusion: From October 2010 to March 2012, pandemic strains of pandemic influenza A (H1N1) and seasonal H3N2 in Jinzhou City were high prevalence strains. The positive rate of flu was high in middle-aged and elderly people, and the monitoring of the elderly should be strengthened to detect the virus mutation in time .