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目的 :观察癫大鼠海马组织超氧化物歧化酶 (SOD)与海马一氧化氮 (NO)的变化 ,并探讨二者之间的关系。 方法 :通过贝美格制成癫发作动物模型 ,分别取发作 5、30min及注药 6、12、2 4h的海马组织 ,硝酸还原酶法、黄嘌呤氧化酶法分别测NO、SOD。 结果 :海马组织SOD于发作 5min时显著低于正常对照组 ;6h时仍与对照组存在差异 ;12h后差异不显著 ;海马组织NO在发作 5min迅速升高 ,30min组仍高于正常对照组 ,6h后与正常对照组比无显著差异。经相关统计学检验 ,SOD与NO之间呈显著负相关 (r =- 0 .84 9,P <0 .0 1)。 结论 :癫发作可导致海马自由基清除剂SOD严重损伤 ,SOD活力降低与NO之间存在相关关系
Objective: To observe the changes of superoxide dismutase (SOD) and hippocampal nitric oxide (NO) in hippocampus of rats with epilepsy and to explore the relationship between the two. Methods: The animal model of epileptic seizures was made by Beomes. The hippocampus tissues were taken at 5,30 minutes and 6,12,24 hours respectively. NO and SOD were measured by nitrate reductase and xanthine oxidase respectively. Results: SOD in hippocampus was significantly lower than that in normal control group at 5 min and remained unchanged at 6 h, but not significantly after 12 h. NO in hippocampus increased rapidly at 5 min and remained higher than that in control group at 30 min, After 6h compared with the normal control group no significant difference. Correlation analysis showed that there was a significant negative correlation between SOD and NO (r = - 0.84 9, P <0.01). CONCLUSION: Epileptic seizures can lead to severe damage of SOD in free radical scavenger of hippocampus and decrease of SOD activity and NO