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硫葡萄糖甙总量的测定亦成为油菜品质鉴定的一个重要项目。迄今为止,硫葡萄糖甙分析方法已有几十种,大致可分为五类。第一类为硫酸钡重量法,容量法。这属于常规测试,前者样品用量大,操作费时。后者滴定终点不明显。二者的灵敏度均较低。第二类为测挥发性配糖基的紫外硫脲法。但此法对于哚吲硫甙无响应,因而测定结果往往偏低。第三类为TMS气谱法,此法虽可测定硫甙的组成而进行定量,但应用结果表明,其再现性较差,测定结果难于比较,且成本费用太大。第四类为直接测硫甙的液相色谱法,此法需用昂贵的仪器与药品试剂,操作技术较复杂不宜推广。第五类为测葡萄糖法。其中葡萄糖试纸法只能作半定量测定;紫外分光光度法,其灵敏度虽较高,但未考虑到游
The determination of thioglycoside total amount has also become an important item of rapeseed quality identification. To date, there have been dozens of methods for the analysis of thioglycosides, which can be roughly divided into five categories. The first category is barium sulfate gravimetric method, volumetric method. This is a routine test, the former sample dosage, operation time-consuming. The latter titration end is not obvious. The sensitivity of both are lower. The second category for the determination of volatile glycosides with UV thiourea method. However, this method for indole glucosinolate no response, and thus the results are often low. The third type is TMS gas chromatography. Although this method can determine the composition of glucosinolate and quantify it, the application results show that its reproducibility is poor, the measurement results are difficult to compare, and the cost is too high. The fourth category is the direct measurement of glucosinolate liquid chromatography, this method requires expensive equipment and pharmaceutical reagents, the operation is more complex and should not be promoted. The fifth category for the measurement of glucose method. Which glucose test strip method can only be used for semi-quantitative determination; UV spectrophotometry, although its high sensitivity, but did not consider the tour