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本文对伤湿宝珍膏中麝香有效成分麝香酮的检出方法作了初步研究,摸索了伤湿宝珍膏中麝香酮的提取分离方法,并利用麝香酮苯腙衍生物分子量大,薄层层析时易与其它杂质分离的原理,对供试液和麝香酮标准品溶液分别加入2,4——二硝基苯肼试液,生成桔黄色的麝香酮苯腙,然后在同一块硅胶G烧结板上点样展开,展开剂为甲苯:乙酸乙酯(98:2),展开后,在同-值Rf处,比较供试液和麝香酮苯腙标准品的层折斑点,用以判断供试液中是否含有麝香酮。本法对麝香酮的最小检出量约为0.23μg。同时,利用薄层分离制备了较纯的麝香酮苯腙液,并用紫外分光光度计测定麝香酮苯腙标准品和麝香酮苯腙供试液在紫外区的吸收光谱,得知两者在204nm处都有一强而尖锐的吸收峰,且不受其它杂质影响。据此,可较简便地采用紫外分光光度计直接对供试液中的麝香酮加以检测,并为今后对伤湿宝珍膏中麝香酮的定量分析提供了依据。
In this paper, a preliminary study was made on the detection method of musk ketone, the effective component of musk in Zhenshi Baozheng Ointment, and the method for extraction and separation of musk ketone in Huo Shi Bao Zhen Ointment was explored. The musk ketone phenylhydrazone derivative was used for large molecular weight and thin layer. The principle of separation from other impurities during chromatography is to add 2,4-dinitrophenylhydrazine test solution to the test solution and the solution of muskone standard solution to generate orange musk ketone benzoquinone, and then in the same piece of silica gel The G sintering plate was spotted and the developing agent was toluene:ethyl acetate (98:2). After unrolling, the spotted spots for the test solution and the muskone phenylhydrazine standard were compared at the same value Rf. Determine whether the test solution contains musk ketone. The minimum detectable amount of muskone by this method is approximately 0.23 μg. Simultaneously, a relatively pure muskone phenylhydrazone solution was prepared by thin layer separation, and the absorption spectra of the muskone benzoquinone standard product and the muskone phenylhydrazine test solution in the ultraviolet region were determined by an ultraviolet spectrophotometer, and both were found at 204 nm. There is a strong and sharp absorption peak everywhere and it is not affected by other impurities. According to this, it is easier to use the UV spectrophotometer to directly test the musk ketone in the test solution, and to provide a basis for the quantitative analysis of musk ketone in the Zhenshibao cream.