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目的:探讨转录因子Yin Yang 1(YY1)在正常食管组织和食管鳞癌组织中的表达及其在食管癌发生发展中的作用。方法:免疫组化染色检测15例正常食管组织,39例食管癌旁组织和88例食管癌组织样品中YY1蛋白表达水平;噻唑蓝(MTT)和克隆实验检测食管鳞癌细胞(TE-1细胞)增殖和生长变化;蛋白质印迹(Western blot)技术检测转染后转录因子YY1和p21表达水平;细胞划痕实验和Transwell实验检测TE-1细胞迁移、侵袭能力的变化。结果:与正常食管组织和食管癌旁组织相比,YY1蛋白在食管鳞癌组织样品中的表达显著提高。此外,转染了YY1过表达载体后明显抑制TE-1细胞生长,而干扰YY1表达则可促进细胞的生长;同时伴随着YY1靶基因p21(也称p21WAF1/Cip1)的变化。TE-1细胞转染YY1过表达载体后,细胞的侵袭、迁移能力增强。结论:食管鳞癌组织中YY1表达显著上调,YY1可调节细胞的生长和转移侵袭能力。因此,YY1有望成为食管癌诊断和发展的分子标志物,也有可能是食管癌基因治疗的一个潜在靶点。
Objective: To investigate the expression of transcription factor Yin Yang 1 (YY1) in normal esophageal and esophageal squamous cell carcinoma and its role in the development of esophageal cancer. Methods: The expression of YY1 protein in 15 normal esophageal tissues, 39 adjacent esophageal tissues and 88 esophageal cancer tissues was detected by immunohistochemical staining. The expression of YY1 protein in esophageal squamous carcinoma cells (TE-1 cells) was detected by MTT assay ) Proliferation and growth. The expression of YY1 and p21 were detected by Western blot. The cell migration and invasion ability of TE-1 cells were detected by cell scratch assay and Transwell assay. Results: The expression of YY1 protein in esophageal squamous cell carcinoma tissue samples was significantly higher than that in normal esophageal tissues and esophageal tissues. In addition, transfection of YY1 overexpression vector significantly inhibited the growth of TE-1 cells, while interfering with YY1 expression can promote cell growth; accompanied by YY1 target gene p21 (also known as p21WAF1 / Cip1) changes. TE-1 cells transfected with YY1 overexpression vector, the cell invasion, migration enhanced. Conclusion: The expression of YY1 in esophageal squamous cell carcinoma is significantly up-regulated, and YY1 can regulate the growth and metastasis of esophageal squamous cell carcinoma. Therefore, YY1 is expected to become a molecular marker for the diagnosis and development of esophageal cancer, and may also be a potential target for esophageal cancer gene therapy.