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目的:探讨超声介导微泡破裂法促进血管内皮生长因子(VEGF)基因在糖尿病鼠缺血骨骼肌内转染的作用,评估其转染效率和安全性。方法:建立糖尿病鼠缺血骨骼肌动物模型,以绿色荧光蛋白基因为报告基因,观察接受超声及微泡治疗组h VEGF165基因在糖尿病鼠缺血骨骼肌内表达,并与对照组相比。同时取糖尿病鼠缺血骨骼肌进行HE染色行组织学检查。结果:在超声介导微泡破裂组内,h VEGF165基因表达明显增强(42.87±5.12),与单纯接受质粒治疗组(5.02±1.21)和接受质粒和超声治疗组(8.16±2.43)相比,差异具有统计学意义(P<0.001),HE切片未发现肌组织结构的改变。结论:超声介导微泡破裂法能有效促进外源基因在糖尿病鼠缺血骨骼肌中表达,为糖尿病周围血管疾病的基因治疗提供了实验依据。
OBJECTIVE: To investigate the role of ultrasound-mediated microbubble rupture in promoting the transfection of vascular endothelial growth factor (VEGF) gene in skeletal muscle of diabetic rats and to evaluate its transfection efficiency and safety. Methods: Animal models of skeletal muscle in diabetic mice were established. The expression of h VEGF165 gene in the ischemic skeletal muscle of diabetic mice was observed by using green fluorescent protein gene as reporter gene and compared with the control group. Meanwhile, the ischemic skeletal muscle of diabetic rats was taken for histological examination by HE staining. Results: The expression of h VEGF165 gene was significantly increased in the ultrasound-mediated microbubble rupture group (42.87 ± 5.12) compared with the plasmid-treated group (5.02 ± 1.21) and the plasmid-treated and ultrasound-treated group (8.16 ± 2.43) The difference was statistically significant (P <0.001), HE section did not find changes in muscle structure. CONCLUSIONS: Ultrasound-mediated microbubble rupture can effectively promote the expression of foreign genes in the skeletal muscle of diabetic rats and provide an experimental basis for the gene therapy of peripheral vascular diseases.