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目的：观察体外ＴＮＦα联用ＩＬ－２培养ＬＡＫ细胞的抗胃癌作用及ＴＮＦα预处理后胃癌细胞对ＬＡＫ细胞敏感性的变化。方法：以不同浓度ＴＮＦα与ＩＬ－２配伍培养ＬＡＫ细胞，并做ＴＮＦα的单抗阻断试验，用ＬＤＨ释放法检测ＬＡＫ细胞抗癌活性；以ＴＮＦα预处理胃癌细胞，检测ＬＡＫ细胞抗瘤活性。结果：ＴＮＦα在低浓度ＩＬ－２下增加ＬＡＫ活性，使ＩＬ－２诱导同等ＬＡＫ活性的浓度下降约１０倍；ＴＮＦα预处理使ＬＡＫ对其抗瘤活性提高约９％。结论：ＴＮＦα可增加ＩＬ－２诱导的ＬＡＫ抗胃癌活性；ＴＮＦα预处理可使胃癌细胞对ＬＡＫ的敏感性增加
OBJECTIVE: To observe the anti-cancer effect of LAK cells cultured with TNFα in combination with IL-2 in vitro and the changes of sensitivity of LAK cells to gastric cancer cells treated with TNFα. METHODS: LAK cells were cultured with different concentrations of TNFα and IL-2. LAK monoclonal antibody blocking assay was performed. The anticancer activity of LAK cells was detected by LDH release method. Gastric cancer cells were pretreated with TNFα and the antitumor activity of LAK cells was detected. RESULTS: TNFα increased LAK activity at low concentrations of IL-2, resulting in a 10-fold decrease in the concentration of IL-2 induced equivalent LAK activity; pretreatment with TNFα increased the anti-tumor activity of LAK by approximately 9%. Conclusion: TNFα can increase IL-2-induced anti-gastric activity of LAK; TNFα pretreatment can increase the sensitivity of gastric cancer cells to LAK