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目的:建立测定骆驼刺中总还原糖与芦丁含量的方法,并对不同部位的样品含量进行比较。方法:以硫酸蒽酮法,采用紫外分光光度法测定骆驼刺中总还原糖的含量,以葡萄糖为对照品,检测波长620 nm;采用高效液相色谱法测定芦丁的含量,色谱柱Symmetry Shield Rp-18(3.9 mm×150 mm,5μm),流动相乙腈-0.1%磷酸水溶液(19∶81),检测波长360 nm,柱温30℃。结果:葡萄糖检测的线性范围3.85~70.33 mg·L-1(r=0.995 9),平均加样回收率99.60%,RSD 1.5%(n=6);芦丁检测的线性范围4.95~59.40 mg·L-1(r=0.999 6),平均加样回收率99.30%,RSD 2.08%。结论:不同部位的骆驼刺中芦丁和总还原糖含量有一定差异,其中果实及地上部分中总还原糖和芦丁的含量比较高。该结果可为骆驼刺的种植和采收的研究奠定基础。
Objective: To establish a method for determination of total reducing sugar and rutin in Alhagi sparsifolia, and to compare the contents of different parts of the sample. Methods: The total reducing sugar content of Alhagi sparsifolia was determined by ultraviolet spectrophotometry with sulfuric acid anthrone method. The detection wavelength was 620 nm with glucose as reference substance. The content of rutin was determined by high performance liquid chromatography (HPLC) Rp-18 (3.9 mm × 150 mm, 5 μm), mobile phase acetonitrile-0.1% phosphoric acid solution (19:81), detection wavelength 360 nm and column temperature 30 ℃. Results: The linear range of glucose detection ranged from 3.85 to 70.33 mg · L-1 (r = 0.995 9) with the average recovery of 99.60% and RSD 1.5% (n = 6). The linear range of detection of rutin was 4.95-59.40 mg · L-1 (r = 0.999 6), the average recovery rate of 99.30%, RSD 2.08%. CONCLUSION: The content of rutin and total reducing sugars in Alhagi sparsifolia is different in different parts, and the contents of total reducing sugars and rutin in fruits and aboveground parts are relatively high. The results laid the foundation for the research on the cultivation and harvesting of Alhagi sparsifolia.