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本文报告对257例不同孕周的孕妇血清进行乙肝两对半,AFP—RIA 同步测定,按乙肝两对半检查有无阳性分组,合并孕周进行统计学处理后加以分析。对象和方法一、对象:不同妊娠期的257例孕妇均在本院保健门诊建卡,定期检查至分娩。初诊建卡孕期在12～31周,年龄21～34岁,空腹抽取肘静脉分离血清,冰箱保存待测。二、方法:乙肝两对半采用中国深圳南园生化试剂厂的酶标法试剂盒,上海放射免疫分析技术研究所提供的 AFP 双抗放射免疫试剂盒,使用国营二六二厂生产的 FJ—2003/50Gr 免疫计数器测定,各项实验均严格按照试剂盒说明书操作。
This paper reports 257 pregnant women with different gestational weeks of hepatitis B two pairs of semi-AFP-RIA simultaneous determination of hepatitis B two pairs of semi-check whether positive group, combined with gestational weeks for statistical analysis and analysis. Subjects and methods First, the object: 257 pregnant women in different gestation period in our hospital health clinic card, regular check until delivery. The initial diagnosis of card during pregnancy in 12 to 31 weeks, aged 21 to 34 years old, fasting elbow venous sera were separated, the refrigerator to be tested. Second, methods: Hepatitis B two pairs of half using Shenzhen South Park Biochemical Reagent Factory ELISA Kit, Shanghai Institute of Radioimmunoassay provided AFP double-antibody radioimmunoassay kit, the use of State-run Plant 262 FJ- 2003 / 50Gr immune counter determination, the experiments are in strict accordance with kit instructions.