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Objective;To find out whether ultraviolet ray,a kind of non-ionic ray,could cause the bystander effect,the UV exposed MOLT-4 cells had been investigated.Methods:Two experiment groups were carried out,in which cells were culture and treated at two concentrations:2 x 105/mL and 5 x 105/mL.All other treatments were the same.Part of the cells was labeled with DID and exposed to UV ray for 40 s as effect cells;other cells was untreated as bystander cells.Then,the cells were co-cultured and harvested at 4 h interval over a period of 24 h.Annexin V-FITC/PI assay was used to evaluate the bystander effect in bystander cells co-cultured with effected cells.Laser confocal microscope method was used to observe the morphologic changes of the bystander cells.Results:The percentage of cells undergoing apoptosis in the bystander cells was increased over time compared with the control group.They were 6.84%,8.09%,9.88%,17.64%,17.43%,30.99% and 37.93% respectively in 0 h,4 h,8 h,12 h,16 h,20 h and 24 h.When observed by laser scanning confocal microscope,the bystander cells show some classic character of apoptosis such as chromosome condense,phosphatidylserine transfer and formation of apoptotic bodies.Conclusion:Bystander effect is significant in un-irradiated bystander MOLT-4 cells when co-cultured with UV exposed cells.