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目的探讨miRNA-148b在肾癌组织的表达及临床意义。方法选取2012年3月至2014年3月间潍坊医学院附属医院泌尿外科收治的40例肾癌患者,采用实时定量聚合酶链反应(PCR)检测40例患者肾癌及癌旁正常肾组织中miRNA-148b的表达情况,并研究其与临床分期、病理分级之间的关系。结果与配对的癌旁正常肾组织相比,肾癌组织中miRNA-148b的相对表达下降,差异有显著统计学意义(P<0.01)。不同临床分期和病理分级的肾癌组织中miRNA-148b的相对表达差异无统计学意义(P>0.05)。结论 miR-148b在肾癌组织中的表达水平较癌旁正常肾组织明显降低,但与肾癌的病理分级及临床分期无明显相关性。
Objective To investigate the expression of miRNA-148b in renal cell carcinoma and its clinical significance. Methods Forty patients with renal cell carcinoma admitted to the Department of Urology, Affiliated Hospital of Weifang Medical College from March 2012 to March 2014 were enrolled in this study. Real-time quantitative polymerase chain reaction (PCR) was used to detect 40 patients with renal cell carcinoma and adjacent normal renal tissues miRNA-148b expression, and study its relationship with clinical stage, pathological grade. Results The relative expression of miRNA-148b in renal cell carcinoma was significantly lower than that in paired adjacent normal renal tissues (P <0.01). There was no significant difference in the relative expression of miRNA-148b in different clinical stage and pathological grade of renal cell carcinoma (P> 0.05). Conclusion The expression of miR-148b in renal cell carcinoma tissue was significantly lower than that in normal lung tissue, but not with the pathological grade and clinical stage of renal cell carcinoma.