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目的探讨胸苷酸合成酶(TS)基因多态性与肺癌易感性的关系。方法应用聚合酶链式反应(PCR)方法检测青岛地区原发性肺癌患者473例及健康对照641人的TS 5’-非翻译区(untranslated region,UTR)多态性分布,以非条件logistic回归模型分析TS基因多态性与肺癌易感性的关系。结果 TS 5’-UTR的3种基因型3R/3R、2R/3R、2R/2R在病例组的频率分别为62.2%、34.0%和3.8%,在对照组中则分别为60.5%、37.0%和2.5%,分布差异无统计学意义(P>0.05);以携带TS 5’-UTR 3R/3R基因型者为参照,携带TS 5’-UTR 2R/3R基因型者发生肺癌的风险减少,OR值为0.90(95%CI=0.69～1.17),而携带TS 5’-UTR 2R/2R基因型者发生肺癌风险增加,OR值为1.64(95%CI=0.80～3.38),但差异均无统计学意义(P>0.05);以性别、年龄、吸烟、饮酒、病理类型,临床分期分层分析结果表明,TS 5’-UTR基因多态性与肺癌易感性无明显关联(均P>0.05)。结论 TS 5’-UTR多态与肺癌易感性无显著关联,未发现该基因多态性与吸烟、饮酒存在明显的交互作用。
Objective To investigate the relationship between thymidylate synthase (TS) gene polymorphisms and lung cancer susceptibility. Methods Polymorphism of TS 5’-untranslated region (UTR) in 473 primary lung cancer patients and 641 healthy controls in Qingdao area was detected by polymerase chain reaction (PCR). The non-conditional logistic regression Model analysis of TS gene polymorphism and susceptibility to lung cancer. Results The frequencies of 3R / 3R, 2R / 3R and 2R / 2R in TS 5’-UTR were 62.2%, 34.0% and 3.8% in the case group and 60.5% and 37.0% in the control group respectively And 2.5%, respectively. There was no significant difference in distribution between the two groups (P> 0.05). The risk of developing lung cancer in TS 5’-UTR 2R / 3R genotype was lower than that in TS 5’-UTR 3R / The odds ratio was 0.90 (95% CI = 0.69-1.17), while the risk of lung cancer increased with the TS 5’-UTR 2R / 2R genotype (OR = 1.64, 95% CI = 0.80-3.38) (P> 0.05). Stratified analysis by sex, age, smoking, drinking, pathological type and clinical stage showed that there was no significant correlation between TS 5’-UTR gene polymorphism and lung cancer susceptibility ). Conclusion TS 5’-UTR polymorphism is not associated with susceptibility to lung cancer. No significant interaction between TS 5’-UTR polymorphism and smoking and alcohol consumption has been found.