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OBJECTIVE:Ligusticum porteri is a traditional Native American herb.The roots of L.porteri are traditionally used in the treatment of many diseases,however,its cytotoxicity,antioxidative and immunemodulatory effects need to be investigated.In this study,we evaluated the effects of the root extract at different doses on human peripheral blood lymphocytes(PBLs).METHODS:The lymphocytes were incubated with different concentrations of the root extracts(0,50,100,200,and 400 ug/mL) and harvested every 6 h for 2 d(P<0.05).The protective effect of the herb against oxidative damage was determined by inducing oxidative stress with the administration of50 pmol/L of hydrogen peroxide(H_2O_2).RESULTS:Treatments with L.porteri at 200 and 400 pg/mL increased the viability of PBLs.The deleterious effect of H_2O_2 was ameliorated by 400 ug/mL L.porteri treatment.Addition of 400 pg/mL L.porteri reduced lipid peroxidation in stressed PBLs by 94%(P<0.05).Treatment with 400 μg/mL of L.porteri resulted in a 26.4%increase of reduced glutathione levels.Activities of superoxide dismutase and catalase increased by 17.5%and 55.2%respectively,when stressed PBLs were treated with 400 μg/mL L.porteri for 2 d(P<0.05).Treatment with 400 pg/mL L.porteri increased interferon-γ and interleukin-2expressions in H_2O_2-challenged PBLs(P<0.05),however,the root extract did not cause a significant difference in interleukin-10 levels compared to the control(P>0.05).CONCLUSION:The findings suggest that L.porteri might be a potential immune-modulating agent involving protective effects against oxidative damage.
OBJECTIVE: Ligusticum porteri is a traditional Native American herb. The roots of L. porteri are traditionally used in the treatment of many diseases, however, its cytotoxicity, antioxidative and immune modulatory effects need to be investigated. In this study, we evaluated the effects of the root extracts at different doses on PBLs. METHODS: The lymphocytes were incubated with different concentrations of the root extracts (0, 50, 100, 200 and 400 ug / mL) and harvested every 6 h for 2 d (P < 0.05). The protective effect of the herb against oxidative damage was determined by inducing oxidative stress with the administration of 50 pmol / L of hydrogen peroxide (H 2 O 2) .RESULTS: Treatments with L. porteri at 200 and 400 pg / mL increased the viability of PBLs. The deleterious effect of H_2O_2 was ameliorated by 400 μg / mL L. porteri treatment. Condition of 400 pg / mL L. porteri reduced lipid peroxidation in stressed PBLs by 94% (P <0.05). Treatment with 400 μg / mL of L. porteri resulted in a 26.4% increase of reduced glutathione levels. Activities of superoxide dismutase and catalase increased by 17.5% and 55.2% respectively when stressed PBLs were treated with 400 μg / ml L. porteri for 2 days (P <0.05). Treatment with 400 pg / mL L. porteri increased interferon-γ and interleukin-2 expressions in H_2O_2-challenged PBLs (P <0.05), however, the root extract did not cause a significant difference in interleukin-10 levels compared to the control (P> 0.05) .CONCLUSION : The findings suggest that L. porteri might be a potential immune-modulating agent involving protective effects against oxidative damage.