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目的了解冷冻保存对射出精子和经皮附睾、睾丸微穿刺抽吸精子进行卵胞质内单精子注射(ICSI)临床结果的影响。方法将射出精子152个治疗周期和经皮附睾、睾丸微穿刺抽吸精子55个周期分别分为新鲜组和冷冻组,对其ICSI后的结果进行对照。结果射出精子新鲜组和冷冻组受精率、卵裂率和临床妊娠率(分别为79.70%vs76.79%、97.08%vs98.35%、39.02%vs37.93%)与经皮附睾、睾丸微穿刺抽吸精子新鲜组和冷冻组受精率、卵裂率和临床妊娠率/穿刺周期(分别为77.07%vs74.54%、97.47%vs94.31%、35.00%vs37.14%)均无显著差异(P>0.05)。结论精子的冷冻保存对射出精子和经皮附睾、睾丸微穿刺抽吸精子进行ICSI的临床结果没有影响,我们认为对于经皮附睾、睾丸微穿刺抽吸精子应该进行冷冻保存,以减少对附睾、睾丸的穿刺次数。
Objective To investigate the effect of cryopreservation on the clinical outcome of intracytoplasmic sperm injection (ICSI) by aspiration of spermatozoa and percutaneous epididymis by micro-aspiration of testis. Methods Fifty-two treatment cycles of injection of sperm and percutaneous epididymal aspiration and 55 cycles of testicular micro-aspirate sperm aspiration were divided into fresh group and frozen group, respectively. The results of ICSI were compared. Results The fertilization rate, cleavage rate and clinical pregnancy rate of fresh group and frozen group were 79.70% vs 76.79%, 97.08% vs 98.35%, 39.02% vs 37.93%, respectively. There was no significant difference in fertilization rate, cleavage rate and clinical pregnancy rate / puncture cycle in aspirated sperm fresh group and frozen group (77.07% vs74.54%, 97.47% vs94.31%, 35.00% vs37.14%, respectively) P> 0.05). CONCLUSIONS: Cryopreservation of sperm has no effect on the clinical outcome of sperm injection and percutaneous epididymal sperm aspiration by micro-aspiration of testis and aspiration of spermatozoon on testicular microperitoneal aspiration of spermatozoa in percutaneous epididymis, Testicular puncture times.