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目的分析缺失编码Ⅲ型分泌系统的大质粒p CD1对鼠疫耶尔森菌蛋白质表达谱的影响,探索鼠疫菌的潜在致病机制,为鼠疫疫苗研制提供新线索。方法通过双向电泳与质谱分析相结合的方法,比较鼠疫菌在26℃和37℃不同培养温度下,全菌蛋白表达谱差异,鉴定差异蛋白并对其功能进行初步分析。结果成功分离并鉴定了鼠疫菌201株和201△p CD1缺失突变株中的差异蛋白,其中26℃条件下鉴定到24个差异蛋白,37℃条件下鉴定到25个差异蛋白,内含7个由p CD1质粒编码的蛋白。结论通过比较蛋白质组学研究,发现大质粒p CD1缺失后,多种染色体编码蛋白的丰度发生了显著变化,暗示大质粒能调控染色体编码基因的表达。
Objective To analyze the influence of the large plasmid pCD1 encoding type Ⅲ secretion system on Yersinia pestis protein expression profiles and to explore the potential pathogenesis of Yersinia pestis and to provide new clues for the development of plague vaccine. Methods By means of two-dimensional electrophoresis and mass spectrometry, the differences of expression profiles of whole bacteria proteins from Yersinia pestis at 26 ℃ and 37 ℃ were compared, and the differential proteins were identified and their functions were analyzed preliminarily. Results The differential proteins of 201 strains of Y. pestis and 201 △ p CD1 deletion mutant were successfully isolated and identified. Among them, 24 differential proteins were identified at 26 ℃, 25 differential proteins were identified at 37 ℃, including 7 The protein encoded by the pCD1 plasmid. Conclusion By comparing proteomics studies, it was found that the abundance of multiple chromosomally encoded proteins varied significantly after deletion of the large plasmid pCD1, suggesting that large plasmids could regulate the expression of chromosomally encoded genes.