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为了建立须癣毛癣菌快速诊断方法,采集疑似患真菌病的家兔皮屑样品96份,制作涂片,分别进行直接镜检、G rocott六胺银染色、过碘酸雪夫氏染色、Calcofluor W hite荧光染色和定量PCR检测。结果显示,4种常规方法的阳性率依次为69.78%、71.19%、72.92%和84.38%,特异性和阳性预测值没有显著差异,而荧光染色检查的敏感性和阴性预测值明显高于其他3常规种方法(P<0.01)。定量PCR的阳性率略高于荧光染色,但没有显著差异。与其他3种常规方法相比,Calcofluor White荧光染色方法相对简单、快捷、敏感、阳性率高,其阳性率与定量PCR相当,但费用低。由此可见,Calcofluor White荧光染色方法是须癣毛癣菌感染可选择的检测方法。
In order to establish a rapid diagnosis method of Trichophyton mentagrophytes, 96 samples of rabbit dander suspected to suffer from mycosis were collected and smeared. The specimens were directly examined by microscopy, Gottocott silver staining, periodic acid Schiff’s staining, Calcofluor W hite fluorescent staining and quantitative PCR detection. The results showed that the positive rate of the four routine methods were 69.78%, 71.19%, 72.92% and 84.38%, the specificity and positive predictive value was not significantly different, while the sensitivity and negative predictive value of fluorescent staining was significantly higher than the other three Conventional methods (P <0.01). The positive rate of quantitative PCR slightly higher than the fluorescence staining, but no significant difference. Compared with the other three conventional methods, Calcofluor White fluorescence staining method is relatively simple, fast, sensitive, positive rate, the positive rate and quantitative PCR equivalent, but low cost. Thus, Calcofluor White staining method is an alternative method of detection of Trichophyton mentagrophytes infection.