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目的：了解全反式维甲酸（ＡＴＲＡ）对ＣＮＥ－２Ｚ细胞增殖生长及凋亡有无影响。方法：采用ＭＴＴ法、细胞核染色、ＤＮＡ裂解率及电泳分析。结果：（１）用ＡＴＲＡ诱导细胞至７２ｈ，细胞增殖抑制率随浓度升高而增加。（２）在终浓度为５×１０－６ｍｏｌ／Ｌ诱导ＣＮＥ－２Ｚ细胞５天，可见部分细胞体积变小，核浓缩及核碎裂；ＤＮＡ裂解率（４４．２０±６．３２）明显高于对照组（１５．４１±５．５０），差别有显著性，Ｐ＜０．０１；ＤＮＡ琼脂糖电泳，出现不连续的典型梯状ＤＮＡ条带。片段大小为２００ｂｐ或其倍数。（３）在浓度为５×１０－７ｍｏｌ／Ｌ诱导５天和５×１０－６ｍｏｌ／Ｌ诱导４天时，均无明显细胞凋亡出现。结论：ＡＴＲＡ对ＣＮＥ－２Ｚ细胞增殖有抑制作用，且随浓度增加而增强；ＡＴＲＡ可诱导ＣＮＥ－２Ｚ细胞发生凋亡，但表现出明显的剂量和时间依赖性。
OBJECTIVE: To investigate the effects of all-trans retinoic acid (ATRA) on the proliferation and apoptosis of CNE-2Z cells. Methods: MTT assay, nuclear staining, DNA cleavage rate and electrophoresis analysis were used. RESULTS: (1) When cells were induced with ATRA for 72 h, the inhibition rate of cell proliferation increased with increasing concentrations. (2) After 5 days of induction of CNE-2Z cells at a final concentration of 5×10-6 mol/L, part of the cell volume became smaller, nuclear condensation and nuclear fragmentation were observed, and the DNA fragmentation rate was significantly higher (44.20±6.32). In the control group (15.41±5.50), the difference was significant (P<0.01); DNA agarose electrophoresis showed discontinuous typical ladder DNA bands. The fragment size is 200 bp or multiples thereof. (3) There was no obvious cell apoptosis when the concentration was 5×10-7 mol/L for 5 days and 5×10-6 mol/L for 4 days. Conclusion: ATRA can inhibit the proliferation of CNE-2Z cells and increase with the increase of the concentration. ATRA can induce the apoptosis of CNE-2Z cells, but it shows obvious dose and time dependence.