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利用室内选育的敏感品系和抗杀虫双品系为亲本,采用剂量对数—死亡机率值回归线(LD-P线)分析法,研究了小菜蛾对杀虫双的抗性遗传方式。结果表明,小菜蛾对杀虫双的抗性为多基因、常染色体遗传,正、反交F_1的显性度(D)值分别为0.39、0.28,即其主效基因为不完全显性。小菜蛾对杀虫双的抗性现实遗传力较低,h~2=0.052,产生抗性的速率较慢,室内选育119代,抗性仅达122.8倍。抗杀虫双品系和遗传杂交后代(F_1、F_2、BC)对拟除虫菊酯类、氨基甲酸酯类、有机磷类的代表杀虫剂溴氰菊酯、灭多威、敌敌畏等的交互抗性测定结果表明,它们对3种杀虫剂无交互抗性;亲本和杂交后代的多功能氧化酶环氧化活性与杀虫双的抗性水平呈正相关性;乙酰胆碱酯酶活性要比敏感品系低;羧酸酯酶活性与敏感品系无明显差异。
Insecticidal double-resistance inheritance patterns of diamondback moth (Plutella xylostella) were studied by using the logarithm-death chance value regression (LD-P line) analysis method. The results showed that the resistance of diamondback moth to multiple insecticides was polygenic and the autosomal was inherited. The dominant (D) values of positive and negative F_1 were 0.39 and 0.28, respectively, indicating that the major gene was imperfectly dominant. Plutella xylostella resistant to insecticidal double resistance heritability lower, h ~ 2 = 0.052, the rate of resistance is slow, indoor breeding 119 generations, the resistance was only 122.8 times. Anti-insecticidal double-line and cross-breeding offspring (F_1, F_2, BC) were resistant to the interaction of pyrethroids, carbamates, representative insecticides deltamethrin, methomyl, dichlorvos, etc. The results showed that they had no cross resistance to the three insecticides. The epoxidation of multifunctional oxidase between parents and hybrids was positively correlated with the resistance to insecticides. The activity of acetylcholinesterase was lower than that of the susceptible strain ; Carboxylesterase activity and sensitive strains no significant difference.