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目的探讨p16、p53及Ki-67在不同程度宫颈上皮内病变诊断中的临床病理意义。方法选取2012年1月至2014年6月期间成都市妇女儿童中心医院150例患有宫颈鳞癌、宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)以及正常宫颈的组织,用免疫组化SP法检测其p16、p53及Ki-67表达。结果 p16、p53、Ki-67在宫颈鳞癌组织中的阳性表达率分别为100%、46.67%、100%,在CINⅢ中的阳性表达率分别为95%、27.5%、100%,在CINⅡ中的阳性表达率分别为87.5%、22.5%、100%,在CINⅠ中的阳性表达率分别为63.5%、13.33%、100%。宫颈鳞癌组、CIN组(宫颈上皮内病变)中p16、p53及Ki-67的阳性表达率与对照组比较,差异均有统计学意义(P<0.05)。p16、p53及Ki-67在CINⅠ、CINⅡ/Ⅲ及宫颈鳞癌的表达强度逐渐增加,且与CIN分级呈正相关。结论 p16、p53及Ki-67这3种相关蛋白作为辅助的诊断标记物对宫颈病变的诊治有参考价值。
Objective To investigate the clinicopathological significance of p16, p53 and Ki-67 in the diagnosis of cervical intraepithelial lesion. Methods 150 patients with cervical squamous cell carcinoma, cervical intraepithelial neoplasia (CIN) and normal cervix from January 2012 to June 2014 in Chengdu Women and Children's Hospital were enrolled in this study. Immunohistochemical SP method The expressions of p16, p53 and Ki-67 were detected. Results The positive rates of p16, p53 and Ki-67 in cervical squamous cell carcinoma were 100%, 46.67% and 100%, respectively. The positive rates of p16, p53 and Ki-67 in CINⅢ were 95%, 27.5% and 100% The positive rates were 87.5%, 22.5% and 100%, respectively. The positive rates in CINⅠ were 63.5%, 13.33% and 100% respectively. The positive rates of p16, p53 and Ki-67 in cervical squamous cell carcinoma and CIN group (cervical intraepithelial lesion) were significantly different from those in control group (P <0.05). The expressions of p16, p53 and Ki-67 in CINⅠ, CINⅡ / Ⅲand cervical squamous cell carcinoma were gradually increased, and positively correlated with the grade of CIN. Conclusion The three related proteins of p16, p53 and Ki-67 are useful as diagnostic markers for the diagnosis and treatment of cervical lesions.